Journal article
Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens.
- Abstract:
-
Inositol monophosphatase (IMPase) catalyses the hydrolysis of inositol monophosphate to inositol and is crucial in the phosphatidylinositol (PI) signalling pathway. Lithium, which is the drug of choice for bipolar disorder, inhibits IMPase at therapeutically relevant plasma concentrations. Both mouse IMPase 1 (MmIMPase 1) and human IMPase 1 (HsIMPase 1) were cloned into pRSET5a, expressed in Escherichia coli, purified and crystallized using the sitting-drop method. The structures were solved ...
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- Publication status:
- Published
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- Publisher copy:
- 10.1107/s1744309112035191
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Bibliographic Details
- Journal:
- Acta crystallographica. Section F, Structural biology and crystallization communications
- Volume:
- 68
- Issue:
- Pt 10
- Pages:
- 1149-1152
- Publication date:
- 2012-10-01
- DOI:
- EISSN:
-
1744-3091
- ISSN:
-
1744-3091
- Source identifiers:
-
354059
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:354059
- UUID:
-
uuid:3e6665b3-84b4-401d-8863-ac0b846daa46
- Local pid:
- pubs:354059
- Deposit date:
- 2013-11-16
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- Copyright date:
- 2012
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