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Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens.

Abstract:

Inositol monophosphatase (IMPase) catalyses the hydrolysis of inositol monophosphate to inositol and is crucial in the phosphatidylinositol (PI) signalling pathway. Lithium, which is the drug of choice for bipolar disorder, inhibits IMPase at therapeutically relevant plasma concentrations. Both mouse IMPase 1 (MmIMPase 1) and human IMPase 1 (HsIMPase 1) were cloned into pRSET5a, expressed in Escherichia coli, purified and crystallized using the sitting-drop method. The structures were solved ...

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Publication status:
Published

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Publisher copy:
10.1107/s1744309112035191

Authors


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Journal:
Acta crystallographica. Section F, Structural biology and crystallization communications
Volume:
68
Issue:
Pt 10
Pages:
1149-1152
Publication date:
2012-10-01
DOI:
EISSN:
1744-3091
ISSN:
1744-3091
Source identifiers:
354059
Language:
English
Keywords:
Pubs id:
pubs:354059
UUID:
uuid:3e6665b3-84b4-401d-8863-ac0b846daa46
Local pid:
pubs:354059
Deposit date:
2013-11-16

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