Journal article

Multiplexed analysis of chromosome conformation at vastly improved sensitivity

Abstract:: Methods for analyzing chromosome conformation in mammalian cells are either low resolution or low throughput and are technically challenging. In next-generation (NG) Capture-C, we have redesigned the Capture-C method to achieve unprecedented levels of sensitivity and reproducibility. NG Capture-C can be used to analyze many genetic loci and samples simultaneously. High-resolution data can be produced with as few as 100,000 cells, and single-nucleotide polymorphisms can be used to generate allele-specific tracks. The method is straightforward to perform and should greatly facilitate the investigation of many questions related to gene regulation as well as the functional dissection of traits examined in genome-wide association studies.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Davies, J., Telenius, J., McGowan, S., Roberts, N., Taylor, S., Higgs, D., & Hughes, J. (2015). Multiplexed analysis of chromosome conformation at vastly improved sensitivity. Nature Methods, 13(1), 74–80.

MLA Style

Davies, J., et al. “Multiplexed Analysis of Chromosome Conformation at Vastly Improved Sensitivity.” Nature Methods, vol. 13, no. 1, Nature Research, 2015, pp. 74–80.

Chicago Style

Davies, J, J Telenius, S McGowan, N Roberts, S Taylor, D Higgs, and J Hughes. 2015. “Multiplexed Analysis of Chromosome Conformation at Vastly Improved Sensitivity.” Nature Methods 13 (1): 74–80.
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Files:: Davies et al, Multiplexed analysis of chromosome conformation...

(Preview, Accepted manuscript, pdf, 11.5MB, Terms of use)

Publisher copy:: 10.1038/nmeth.3664

Authors

+ Davies, J More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: Weatherall Insti. of Molecular Medicine
Role:: Author

+ Telenius, J More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: Weatherall Insti. of Molecular Medicine
Role:: Author

+ McGowan, S More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: Weatherall Insti. of Molecular Medicine
Role:: Author

+ Roberts, N More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: Weatherall Insti. of Molecular Medicine
Role:: Author

+ Taylor, S More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: Weatherall Insti. of Molecular Medicine
Role:: Author

More authors...

+ Medical Research Council More from this funder

Grant:: 4050189188

+ Wellcome Trust More from this funder

Grant:: 106130/Z/14/Z

Publisher:: Nature Research
Journal:: Nature methods More from this journal
Volume:: 13
Issue:: 1
Pages:: 74-80
Publication date:: 2015-11-23
Acceptance date:: 2015-10-26
DOI:: 10.1038/nmeth.3664
EISSN:: 1548-7105
ISSN:: 1548-7091

Language:: English
Pubs id:: pubs:579451
UUID:: uuid:3e47a1e7-9767-4fb1-93fe-199a788f3543
Local pid:: pubs:579451
Source identifiers:: 579451
Deposit date:: 2016-03-07

Terms of use

Copyright holder:: Nature America
Copyright date:: 2015
Notes:: © 2016 Nature America, Inc. All rights reserved. This is the accepted manuscript version of the article. The final version is available online from Nature Research at: 10.1038/nmeth.3664

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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