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Coarse-grained modelling of DNA-RNA hybrids

Abstract:
We introduce oxNA, a new model for the simulation of DNA-RNA hybrids which is based on two previously developed coarse-grained models—oxDNA and oxRNA. The model naturally reproduces the physical properties of hybrid duplexes including their structure, persistence length and force-extension characteristics. By parameterising the DNA-RNA hydrogen bonding interaction we fit the model's thermodynamic properties to experimental data using both average-sequence and sequence-dependent parameters. To demonstrate the model's applicability we provide three examples of its use—calculating the free energy profiles of hybrid strand displacement reactions, studying the resolution of a short R-loop and simulating RNA-scaffolded wireframe origami.
Publication status:
Published
Peer review status:
Not peer reviewed

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Publisher copy:
10.48550/arxiv.2311.07709

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Role:
Author
ORCID:
0000-0002-3876-0190
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Oxford college:
Queen's College
Role:
Author
ORCID:
0000-0002-2226-9524
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Theoretical Physics
Role:
Author
ORCID:
0000-0002-8438-910X


Host title:
arXiv
Pages:
1-15
Publication date:
2023-11-13
DOI:


Language:
English
Keywords:
Pubs id:
1565691
Local pid:
pubs:1565691
Deposit date:
2024-03-18

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