Journal article icon

Journal article

Rapid and efficient DNA strand cross-linking by click chemistry.

Abstract:
Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much higher thermal stabilities than non-cross-linked ones, with increases in melting temperature of up to 30 degrees C. In some cases, the conversion was near-quantitative, and the reaction was complete in 5 min.
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1002/cbic.200800006

Authors


Kocalka, P More by this author
El-Sagheer, AH More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Journal:
Chembiochem : a European journal of chemical biology
Volume:
9
Issue:
8
Pages:
1280-1285
Publication date:
2008-05-05
DOI:
EISSN:
1439-7633
ISSN:
1439-4227
URN:
uuid:3c8f401e-dcdc-4bc9-9702-7c0e809f728d
Source identifiers:
400015
Local pid:
pubs:400015

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP