Rapid and efficient DNA strand cross-linking by click chemistry.

Journal article

Abstract:: Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much higher thermal stabilities than non-cross-linked ones, with increases in melting temperature of up to 30 degrees C. In some cases, the conversion was near-quantitative, and the reaction was complete in 5 min.

Journal:: Chembiochem : a European journal of chemical biology More from this journal
Volume:: 9
Issue:: 8
Pages:: 1280-1285
Publication date:: 2008-05-01
DOI:: 10.1002/cbic.200800006
EISSN:: 1439-7633
ISSN:: 1439-4227

Language:: English
Keywords:: Oligonucleotides

Electrophoresis, Capillary

Circular Dichroism

DNA

Deoxyuridine

Time Factors

Azides

Alkynes

Molecular Structure

Cross-Linking Reagents
Pubs id:: pubs:400015
UUID:: uuid:3c8f401e-dcdc-4bc9-9702-7c0e809f728d
Local pid:: pubs:400015
Source identifiers:: 400015
Deposit date:: 2013-11-16
ARK identifier:: ark:/29072/ora_3c8f401edcdc4bc997027c0e809f728d

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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