Journal article
CRISPR/Cas precision: do we need to worry about off-targeting in plants?
- Abstract:
- The CRISPR/Cas technology has recently become the tool of choice for targeted genome modification in plants and beyond. Although CRSIPR/Cas offers a rapid and facile way of introducing changes at genomic loci of interest, its application is associated with off-targeting, i.e. introduction of unintended mutations at off-target sites within the genome, which has been reported frequently in the mammalian field. Here we summarise the current knowledge on the precision of CRISPR/Cas in plant systems and provide a summary of state-of the-art strategies for avoiding off-target mutations, as well as unintended on-target changes, in plants. These include using natural (e.g. Cas12a) or engineered (e.g. SpCas9-HF) CRISPR/Cas nucleases characterised by higher precision, as compared to the commonly used wild type SpCas9. In addition, we discuss the usage of CRISPR/Cas nucleases in the form of ribonucleoproteins (RNPs) as an option for reducing off-targeting in plants. Finally, we conclude that the most important factor for reducing CRISPR/Cas off-targeting remains careful selection of target sequences, for which we provide an overview of available online software tools and experimental guidance.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, 745.6KB, Terms of use)
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- Publisher copy:
- 10.1007/s00299-018-2355-9
Authors
- Publisher:
- Springer Nature
- Journal:
- Plant Cell Reports More from this journal
- Volume:
- 38
- Issue:
- 4
- Pages:
- 437-441
- Place of publication:
- Germany
- Publication date:
- 2018-11-13
- Acceptance date:
- 2018-11-07
- DOI:
- EISSN:
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1432-203X
- ISSN:
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1432-203X
- Pmid:
-
30426198
- Language:
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English
- Keywords:
- Pubs id:
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1139953
- Local pid:
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pubs:1139953
- Deposit date:
-
2020-10-26
Terms of use
- Copyright holder:
- Hahn et al.
- Copyright date:
- 2018
- Rights statement:
- ©2018, The Author(s)
- Notes:
- Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Licence:
- CC Attribution (CC BY)
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