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Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine

Abstract:

We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromode-oxyuridine. Low concentrations of EdU (1μM) are sufficient to allow detection of incorporation in cells expressing thymidine kinase and human equilibrative nucleoside transporter 1 (hENT1). However EdU is toxic and activates the rad3-dependent checkpoint, resulting in cell cycle arrest, potentially limiting ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1093/nar/gkr063

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Institution:
University of Oxford
Department:
Mathematical,Physical & Life Sciences Division - Zoology
More by this author
Institution:
University of Oxford
Department:
Mathematical,Physical & Life Sciences Division - Zoology
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Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic Acids Research Journal website
Volume:
39
Issue:
9
Pages:
Article: e60
Publication date:
2011
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:3be6e4cf-dbdb-4938-838a-147d280c4d59
Local pid:
ora:5692
Language:
English
Keywords:
Subjects:

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