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Imaging of single dye-labeled chemotaxis proteins in live bacteria using electroporation

Abstract:

For the last 2 decades, the use of genetically fused fluorescent proteins (FPs) has greatly contributed to the study of chemotactic signaling in E. coli, including the activation of the response regulator protein CheY and its interaction with the flagellar motor. However, this approach suffers from a number of limitations, both biological and biophysical. For example, not all fusions are fully functional when fused to a bulky FP, which can have a similar molecular weight to its fused counterp...

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Publication status:
Published
Peer review status:
Peer reviewed

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Institution:
University of Oxford
Division:
MPLS Division
Department:
Physics; Condensed Matter Physics
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Physics; Condensed Matter Physics
Oxford college:
St Catherines College
Publisher:
Humana Press Publisher's website
Volume:
1729
Pages:
233-246
Series:
Methods in Molecular Biology
Host title:
Bacterial Chemosensing
Publication date:
2018-02-11
DOI:
ISSN:
1064-3745
Pubs id:
pubs:826223
URN:
uri:3be59432-20c8-4524-833a-c2db97893c70
UUID:
uuid:3be59432-20c8-4524-833a-c2db97893c70
Local pid:
pubs:826223
ISBN:
978-1-4939-7576-1

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