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IgG1 pan-neurofascin antibodies identify a severe yet treatable neuropathy with a high mortality

Abstract:
Objectives We aimed to define the clinical and serological characteristics of pan-neurofascin antibody-positive patients.
Methods We tested serum from patients with suspected immune-mediated neuropathies for antibodies directed against nodal/paranodal protein antigens using a live cell-based assay and solid-phase platform. The clinical and serological characteristics of antibody-positive and seronegative patients were then compared. Sera positive for pan-neurofascin were also tested against live myelinated human stem cell-derived sensory neurons for antibody binding.
Results Eight patients with IgG1-subclass antibodies directed against both isoforms of the nodal/paranodal cell adhesion molecule neurofascin were identified. All developed rapidly progressive tetraplegia. Cranial nerve deficits (100% vs 26%), autonomic dysfunction (75% vs 13%) and respiratory involvement (88% vs 14%) were more common than in seronegative patients. Four patients died despite treatment with one or more modalities of standard immunotherapy (intravenous immunoglobulin, steroids and/or plasmapheresis), whereas the four patients who later went on to receive the B cell-depleting therapy rituximab then began to show progressive functional improvements within weeks, became seronegative and ultimately became functionally independent.
Conclusions IgG1 pan-neurofascin antibodies define a very severe autoimmune neuropathy. We urgently recommend trials of targeted immunotherapy for this serologically classified patient group.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1136/jnnp-2021-326343

Authors


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Role:
Author
ORCID:
0000-0002-2329-5708
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Institution:
University of Oxford
Division:
MSD
Department:
Clinical Neurosciences
Role:
Author


Publisher:
BMJ Publishing Group
Journal:
Journal of Neurology, Neurosurgery and Psychiatry More from this journal
Volume:
92
Issue:
10
Pages:
1089-1095
Place of publication:
England
Publication date:
2021-08-16
Acceptance date:
2021-08-09
DOI:
EISSN:
1468-330X
ISSN:
0022-3050
Pmid:
34400540


Language:
English
Keywords:
Pubs id:
1192233
Local pid:
pubs:1192233
Deposit date:
2021-09-08

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