Journal article
Salmonella Typhi and Salmonella Paratyphi A elaborate distinct systemic metabolite signatures during enteric fever
- Abstract:
- The host–pathogen interactions induced by Salmonella Typhi and Salmonella Paratyphi A during enteric fever are poorly understood. This knowledge gap, and the human restricted nature of these bacteria, limit our understanding of the disease and impede the development of new diagnostic approaches. To investigate metabolite signals associated with enteric fever we performed two dimensional gas chromatography with time-of-flight mass spectrometry (GCxGC/TOFMS) on plasma from patients with S. Typhi and S. Paratyphi A infections and asymptomatic controls, identifying 695 individual metabolite peaks. Applying supervised pattern recognition, we found highly significant and reproducible metabolite profiles separating S. Typhi cases, S. Paratyphi A cases, and controls, calculating that a combination of six metabolites could accurately define the etiological agent. For the first time we show that reproducible and serovar specific systemic biomarkers can be detected during enteric fever. Our work defines several biologically plausible metabolites that can be used to detect enteric fever, and unlocks the potential of this method in diagnosing other systemic bacterial infections.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.1MB, Terms of use)
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- Publisher copy:
- 10.7554/eLife.03100.001
Authors
+ Royal Society
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- Funder identifier:
- https://ror.org/03wnrjx87
- Grant:
- 100087/Z/12/Z
+ Wellcome Trust
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- Funder identifier:
- https://ror.org/029chgv08
- Grant:
- 100087/Z/12/Z
+ Swedish Research Council
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- Funder identifier:
- https://ror.org/03zttf063
- Grant:
- VR-NT 2010-4284
- Publisher:
- eLife Sciences Publications
- Journal:
- eLife More from this journal
- Volume:
- 2014
- Issue:
- 3
- Article number:
- e03100
- Publication date:
- 2014-06-05
- Acceptance date:
- 2014-05-26
- DOI:
- EISSN:
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2050-084X
- ISSN:
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2050-084X
- Language:
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English
- Pubs id:
-
476820
- UUID:
-
uuid:3a35aa0a-8be0-4c34-95f9-df272e8d40f0
- Local pid:
-
pubs:476820
- Source identifiers:
-
476820
- Deposit date:
-
2014-08-06
- ARK identifier:
Terms of use
- Copyright holder:
- Näsström et al.
- Copyright date:
- 2014
- Rights statement:
- © Näsström et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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