Targeted transfection by femtosecond laser.

Journal article

The challenge for successful delivery of foreign DNA into cells in vitro, a key technique in cell and molecular biology with important biomedical implications, is to improve transfection efficiency while leaving the cell's architecture intact. Here we show that a variety of mammalian cells can be directly transfected with DNA without perturbing their structure by first creating a tiny, localized perforation in the membrane using ultrashort (femtosecond), high-intensity, near-infrared laser pu...

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Authors: Tirlapur, U; König, K

• Journal: Nature
• Volume: 418
• Issue: 6895
• Pages: 290-291
• Publication date: 2002-07-18
• DOI: 10.1038/418290a
• ISSN: 0028-0836
• Language: English
• Keywords: Cell Line; DNA; Gene Expression; Vaccines, DNA; Infrared Rays; Cell Membrane; Luminescent Proteins; Green Fluorescent Proteins; Transgenes; Genes, Reporter; Transfection; Cell Survival; Genetic Therapy; Macropodidae; Animals; Cricetinae; Culture Media; Lasers; CHO Cells; Fluorescence