Journal article
Handcuffed antisense oligonucleotides for light-controlled cell-free expression
- Abstract:
- Developing simple methods to silence antisense oligonucleotides (ASOs) using photocages opens up the possibility of precise regulation of biological systems. Here, we have developed a photocaging strategy based on ‘handcuffing’ two ASOs to a protein. Silencing was achieved by divalent binding of two terminally photocleavable biotin-modified ASOs to a single streptavidin. These ‘handcuffed’ oligonucleotides showed a drastic reduction in gene knockdown activity in cell-free protein synthesis and were unlocked through illumination, regaining full activity
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.1MB, Terms of use)
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- Publisher copy:
- 10.1039/d3cc01374j
- Publication website:
- http://pure-oai.bham.ac.uk/ws/files/191798820/d3cc01374j.pdf
Authors
+ Engineering and Physical Sciences Research Council
More from this funder
- Funder identifier:
- 10.13039/501100000266
- Grant:
- EP/L015838/1
- EP/V030434/2
- Publisher:
- Royal Society of Chemistry
- Journal:
- Chemical Communications More from this journal
- Volume:
- 59
- Issue:
- 38
- Pages:
- 5685-5688
- Publication date:
- 2023-05-09
- DOI:
- EISSN:
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1364-548X
- ISSN:
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1359-7345
- Language:
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English
- Keywords:
- Pubs id:
-
1341042
- Local pid:
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pubs:1341042
- Source identifiers:
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W4365794108
- Deposit date:
-
2026-05-07
- ARK identifier:
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Terms of use
- Copyright date:
- 2023
- Licence:
- CC Attribution (CC BY)
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