A cool hybrid approach to the herpesvirus 'life' cycle.

Journal article

Electron cryo tomography (cryoET) is an ideal technique to study virus-host interactions at molecular resolution. Imaging of biological specimens in a frozen-hydrated state assures a close to native environment. Various virus-host cell interactions have been analysed in this way, with the herpesvirus 'life' cycle being the most comprehensively studied. The data obtained were further integrated with fluorescence and soft X-ray cryo microscopy data applied on experimental systems covering a wide range of biological complexity. This hybrid approach combines dynamic with static imaging and spans a resolution range from micrometres to angstroms. Along selected aspects of the herpesvirus replication cycle, we describe dedicated combinations of approaches and how subsequent data integration enables insights towards a functional understanding of the underlying processes.

Authors: Zeev-Ben-Mordehai, T; Hagen, C; Grünewald, K

• Publication status: Published
• Publisher: Elsevier
• Journal: Current opinion in virology
• Volume: 5
• Issue: 1
• Pages: 42-49
• Publication date: 2014-04-01
• DOI: 10.1016/j.coviro.2014.01.008
• EISSN: 1879-6265
• ISSN: 1879-6257
• Language: English
• Keywords: Herpesviridae Infections; Herpesviridae; Electron Microscope Tomography; Animals; Cryoelectron Microscopy; Humans