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Multifaceted approaches including neoglycolipid oligosaccharide microarrays to ligand discovery for malectin.

Abstract:

In this chapter, we describe the key procedures for isolation of the oligosaccharides and the preparation of neoglycolipid probes together with expression of malectin that have enabled the discovery of the highly selective binding of this newly described protein in the endoplasmic reticulum (ER) to a diglucosyl high-mannose N-glycan. This is the first indication of a bioactivity for a diglucosyl high-mannose N-glycan of the type that occurs in the ER of eukaryotic cells and which is an interm...

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Publication status:
Published

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Journal:
Methods in enzymology
Volume:
478
Issue:
C
Pages:
265-286
Publication date:
2010-01-01
DOI:
EISSN:
1557-7988
ISSN:
0076-6879
URN:
uuid:39167c67-9eef-405b-b63c-5ed1c2a42e8e
Source identifiers:
101156
Local pid:
pubs:101156

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