Dual role of CO2/HCO3(-) buffer in the regulation of intracellular pH of three-dimensional tumor growths.

Journal article

Intracellular pH (pH(i)), a major modulator of cell function, is regulated by acid/base transport across membranes. Excess intracellular H(+) ions (e.g. produced by respiration) are extruded by transporters such as Na(+)/H(+) exchange, or neutralized by HCO(3)(-) taken up by carriers such as Na(+)-HCO(3)(-) cotransport. Using fluorescence pH(i) imaging, we show that cancer-derived cell lines (colorectal HCT116 and HT29, breast MDA-MB-468, pancreatic MiaPaca2, and cervical HeLa) extrude acid b...

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Authors: Hulikova, A; Vaughan-Jones, R; Swietach, P

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 286
• Issue: 16
• Pages: 13815-13826
• Publication date: 2011-04-01
• DOI: 10.1074/jbc.m111.219899
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Humans; Hydrogen-Ion Concentration; Neoplasms; Bicarbonates; Spheroids, Cellular; Buffers; Disease Progression; Cell Line, Tumor; Hela Cells; Ions; Diffusion; Carbon Dioxide; Protons; Microscopy, Confocal; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Biological Transport