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Journal article

Combined nucleobase and backbone modifications enhance DNA duplex stability and preserve biocompatibility

Abstract:

DNA strands containing a triazole linkage flanked on its 3′-side by an aminoethylphenoxazine nucleobase analogue (G-clamp) have been prepared by solid-phase synthesis followed by CuAAC-mediated click oligonucleotide ligation. The stability of the doubly modified DNA duplexes and DNA-RNA hybrids is greatly increased, whereas a single base pair mismatch located at or adjacent to the modifications is strongly destabilising, making triazole G-clamp a potent mismatch/point mutation sensor. A DNA s...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1039/c3sc51753e

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
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Grant:
BB/ J001694/1 “Extending the boundaries of nucleic acid chemistry.”
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chemical Science Journal website
Volume:
5
Issue:
1
Pages:
253-259
Publication date:
2014-01-01
Acceptance date:
2013-10-14
DOI:
EISSN:
2041-6539
ISSN:
2041-6520
Pubs id:
pubs:443633
UUID:
uuid:380686fb-d3a2-4de7-aa21-ae8af12d9928
Local pid:
pubs:443633
Source identifiers:
443633
Deposit date:
2016-03-01

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