Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.

Journal article

Many protein kinases are activated by a conserved regulatory step involving T-loop phosphorylation. Although there is considerable focus on kinase activator proteins, the importance of specific T-loop phosphatases reversing kinase activation has been underappreciated. We find that the protein phosphatase 6 (PP6) holoenzyme is the major T-loop phosphatase for Aurora A, an essential mitotic kinase. Loss of PP6 function by depletion of catalytic or regulatory subunits interferes with spindle for...

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Authors: Zeng, K; Bastos, R; Barr, F; Gruneberg, U

• Publication status: Published
• Journal: Journal of cell biology
• Volume: 191
• Issue: 7
• Pages: 1315-1332
• Publication date: 2010-12-01
• DOI: 10.1083/jcb.201008106
• EISSN: 1540-8140
• ISSN: 0021-9525
• Language: English
• Keywords: Microtubule-Associated Proteins; Nuclear Matrix-Associated Proteins; Telophase; Chromosome Segregation; Anaphase; Aurora Kinases; Fibroblasts; Kinesin; Phosphorylation; Phosphoprotein Phosphatases; RNA, Small Interfering; Antigens, Nuclear; Tubulin; Protein Subunits; Azepines; Phosphoric Monoester Hydrolases; Humans; Protein Kinase Inhibitors; Cyclin B; Histones; Spindle Apparatus; Nuclear Proteins; Proto-Oncogene Proteins; Mutation; Cell Cycle Proteins; Holoenzymes; Mitosis; Cell Nucleus; Pyrimidines; Cell Line; Catalytic Domain; Models, Biological; Protein-Serine-Threonine Kinases; Protein Binding; Hela Cells