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Design and assembly of double-crossover linear arrays of micrometre length using rolling circle replication

Abstract:
We demonstrate the use of rolling circle replication to template linear DNA arrays whose sizes bridge the gap between nanometre-scale self-assembly and top-down lithographic fabrication. Using rolling circle replication we have produced an oligonucleotide containing several hundred repeats of a short sequence motif. On this template we have constructed, by self-assembly, an array consisting of two parallel duplexes periodically linked by antiparallel Holliday junctions. We have observed arrays up to 10 νm in length by atomic force microscopy. © 2005 IOP Publishing Ltd.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1088/0957-4484/16/9/028

Authors


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Institution:
University of Oxford
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Role:
Author
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Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Condensed Matter Physics
Role:
Author



Publisher:
IOP Science
Journal:
NANOTECHNOLOGY More from this journal
Volume:
16
Issue:
9
Pages:
1574-1577
Publication date:
2005-09-01
DOI:
EISSN:
1361-6528
ISSN:
0957-4484


Pubs id:
pubs:14440
UUID:
uuid:366c8649-4415-483e-bb6a-c1d3124c4ec9
Local pid:
pubs:14440
Source identifiers:
14440
Deposit date:
2013-03-20

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