Journal article
Electroformation of giant unilamellar vesicles on stainless steel electrodes
- Abstract:
- Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electro-swelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively mono-disperse, defect-free vesicles. Currently, however, it is relatively expensive and time-consuming compared to other methods. In this study, we demonstrate that 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 3.5MB, Terms of use)
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- Publisher copy:
- 10.1021/acsomega.6b00395
Authors
+ Marie Curie Intra-European Fellowships
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- Funding agency for:
- Sezgin, E
- Grant:
- MEMBRANE DYNAMICS
+ European Molecular Biology Organization
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- Funding agency for:
- Sezgin, E
- Grant:
- MEMBRANE DYNAMICS
+ Wellcome Trust
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- Funding agency for:
- Sezgin, E
- Eggeling, C
- Grant:
- MEMBRANE DYNAMICS
- 104924/14/Z/14
+ Biotechnology and Biological Sciences Research Council
More from this funder
- Funding agency for:
- Sezgin, E
- Eggeling, C
- Grant:
- MEMBRANE DYNAMICS
- 104924/14/Z/14
+ Engineering and Physical Sciences Research Council
More from this funder
- Funding agency for:
- Sezgin, E
- Eggeling, C
- Grant:
- MEMBRANE DYNAMICS
- 104924/14/Z/14
- EP/I021795/1
- Publisher:
- American Chemical Society
- Journal:
- ACS Omega More from this journal
- Volume:
- 2
- Issue:
- 3
- Pages:
- 994–1002
- Publication date:
- 2017-03-01
- Acceptance date:
- 2017-03-03
- DOI:
- EISSN:
-
2470-1343
- Keywords:
- Pubs id:
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pubs:684020
- UUID:
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uuid:35dac811-ab92-4b12-aaa7-ee38fb22c1a1
- Local pid:
-
pubs:684020
- Deposit date:
-
2017-03-04
- ARK identifier:
Terms of use
- Copyright holder:
- American Chemical Society
- Copyright date:
- 2017
- Notes:
- © 2017 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
- Licence:
- CC Attribution (CC BY)
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