Journal article
Differences in PfEMP1s recognized by antibodies from patients with uncomplicated or severe malaria.
- Abstract:
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Background
Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) variants are encoded by var genes and mediate pathogenic cytoadhesion and antigenic variation in malaria. PfEMP1s can be broadly divided into three principal groups (A, B and C) and they contain conserved arrangements of functional domains called domain cassettes. Despite their tremendous diversity there is compelling evidence that a restricted subset of PfEMP1s is expressed in severe disease. In this study antibodies from patients with severe and uncomplicated malaria were compared for differences in reactivity with a range of PfEMP1s to determine whether antibodies to particular PfEMP1 domains were associated with severe or uncomplicated malaria.
Methods
Parts of expressed var genes in a severe malaria patient were identified by RNAseq and several of these partial PfEMP1 domains were expressed together with others from laboratory isolates. Antibodies from Papuan patients to these parts of multiple PfEMP1 proteins were measured.
Results
Patients with uncomplicated malaria were more likely to have antibodies that recognized PfEMP1 of Group C type and recognized a broader repertoire of group A and B PfEMP1s than patients with severe malaria.
Conclusion
These data suggest that exposure to a broad range of group A and B PfEMP1s is associated with protection from severe disease in Papua, Indonesia.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
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- Files:
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(Preview, Version of record, pdf, 1.0MB, Terms of use)
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- Publisher copy:
- 10.1186/s12936-016-1296-4
Authors
- Publisher:
- BioMed Central
- Journal:
- Malaria Journal More from this journal
- Volume:
- 15
- Issue:
- 1
- Pages:
- 258
- Publication date:
- 2016-05-01
- Acceptance date:
- 2016-04-15
- DOI:
- EISSN:
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1475-2875
- ISSN:
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1475-2875
- Pmid:
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27149991
- Language:
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English
- Keywords:
- Pubs id:
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pubs:619914
- UUID:
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uuid:34efa94e-5402-4c42-848f-b18021a345e0
- Local pid:
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pubs:619914
- Source identifiers:
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619914
- Deposit date:
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2017-12-21
Terms of use
- Copyright holder:
- Duffy et al
- Copyright date:
- 2016
- Notes:
- © 2016 Duffy et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Licence:
- CC Attribution (CC BY)
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