Journal article
RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes.
- Abstract:
- Polycomb group (PcG) proteins function as chromatin-based transcriptional repressors that are essential for normal gene regulation during development. However, how these systems function to achieve transcriptional regulation remains very poorly understood. Here, we discover that the histone H2AK119 E3 ubiquitin ligase activity of Polycomb repressive complex 1(PRC1) is defined by the composition of its catalytic subunits and is highly regulated by RYBP/YAF2-dependent stimulation. In mouse embryonic stem cells, RYBP plays a central role in shaping H2AK119 mono-ubiquitylation at PcG targets and underpins an activity-based communication between PRC1 and Polycomb repressive complex 2(PRC2) which is required for normal histone H3 lysine 27 trimethylation(H3K27me3). Without normal histone modification-dependent communication between PRC1 and PRC2, repressive Polycomb chromatin domains can erode, rendering target genes susceptible to inappropriate gene expression signals. This suggests that activity-based communication and histone modification-dependent thresholds create a localized form of epigenetic memory required for normal PcG chromatin domain function in gene regulation.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 4.6MB, Terms of use)
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- Publisher copy:
- 10.7554/eLife.18591
Authors
+ Lister Institute of Preventive Medicine
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- Funding agency for:
- Klose, R
- Grant:
- 098024/Z/11/Z
+ John Fell Fund, University of Oxford
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- Funding agency for:
- Kessler, B
- Grant:
- 133/075
+ Wellcome Trust
More from this funder
- Funding agency for:
- Kessler, B
- Klose, R
- Grant:
- 133/075
- 098024/Z/11/Z
- Publisher:
- eLife Sciences Publications
- Journal:
- eLife More from this journal
- Publication date:
- 2016-10-05
- Acceptance date:
- 2016-10-01
- DOI:
- EISSN:
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2050-084X
- Language:
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English
- Keywords:
- Pubs id:
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pubs:648298
- UUID:
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uuid:34ba0527-dfd1-42aa-9f2c-716c168d6bf3
- Local pid:
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pubs:648298
- Source identifiers:
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648298
- Deposit date:
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2016-10-07
Terms of use
- Copyright holder:
- © 2016, Rose et al
- Copyright date:
- 2016
- Notes:
- © 2016, Rose et al. Published by eLife Sciences Publications. This article is distributed under the terms of the Creative Commons Attribution License 4.0 permitting unrestricted use and redistribution provided that the original author and source are credited, see: https://creativecommons.org/licenses/by/4.0/
- Licence:
- CC Attribution (CC BY)
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