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Journal article

Expression, limited proteolysis and preliminary crystallographic analysis of IpaD, a component of the Shigella flexneri type III secretion system.

Abstract:

IpaD, the putative needle-tip protein of the Shigella flexneri type III secretion system, has been overexpressed and purified. Crystals were grown of the native protein in space group P2(1)2(1)2(1), with unit-cell parameters a = 55.9, b = 100.7, c = 112.0 A, and data were collected to 2.9 A resolution. Analysis of the native Patterson map revealed a peak at 50% of the origin on the Harker section v = 0.5, suggesting twofold non-crystallographic symmetry parallel to the b crystallographic axis...

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Publication status:
Published

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Publisher copy:
10.1107/s1744309106027047

Authors


Johnson, S More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
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Journal:
Acta crystallographica. Section F, Structural biology and crystallization communications
Volume:
62
Issue:
Pt 9
Pages:
865-868
Publication date:
2006-09-05
DOI:
EISSN:
1744-3091
ISSN:
1744-3091
URN:
uuid:34337bdf-f3fb-4831-b462-e094f46c63eb
Source identifiers:
17026
Local pid:
pubs:17026

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