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Protein-nucleic acid interactions and the expanding role of mass spectrometry.

Abstract:

Mass spectrometry methods are being developed that enable detection of protein interactions with nucleic acids. By mass measuring complexes a direct determination of the stoichiometry of protein-nucleic acid interactions is revealed. For more complex assemblies, using a different approach it is possible to gain information about subcomplexes and even the spatial arrangement of proteins in macromolecular machines. To illustrate these different approaches we review progress and problems encount...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.r300037200

Authors


Hanson, CL More by this author
Robinson, CV More by this author
Journal:
The Journal of biological chemistry
Volume:
279
Issue:
24
Pages:
24907-24910
Publication date:
2004-06-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:33668d7e-0e16-4057-abd7-3055da7bc82a
Source identifiers:
59257
Local pid:
pubs:59257

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