High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe.

Journal article

Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h-, were ordered by hybridizing genetic markers and individual clones from the two libraries. Yeast artificial chromosome (YAC) clones covering the entire genome were used to subdivide the libraries, and hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids provided further mapping information. Restriction...

Expand abstract

Authors: Hoheisel, J; Maier, E; Mott, R; McCarthy, L; Grigoriev, A

• Publication status: Published
• Journal: Cell
• Volume: 73
• Issue: 1
• Pages: 109-120
• Publication date: 1993-04-01
• DOI: 10.1016/0092-8674(93)90164-l
• EISSN: 1097-4172
• ISSN: 0092-8674
• Language: English
• Keywords: Nucleic Acid Hybridization; Base Sequence; Bacteriophage P1; DNA Probes; Repetitive Sequences, Nucleic Acid; Genomic Library; Schizosaccharomyces; Chromosomes, Fungal; Chromosome Mapping; Molecular Sequence Data; Cosmids