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Mean DNA bend angle and distribution of DNA bend angles in the CAP-DNA complex in solution.

Abstract:

In order to define the mean DNA bend angle and distribution of DNA bend angles in the catabolite activator protein (CAP)-DNA complex in solution under standard transcription initiation conditions, we have performed nanosecond time-resolved fluorescence measurements quantifying energy transfer between a probe incorporated at a specific site in CAP, and a complementary probe incorporated at each of five specific sites in DNA. The results indicate that the mean DNA bend angle is 77(+/-3) degrees...

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Publisher copy:
10.1006/jmbi.2001.4976

Authors


Kapanidis, AN More by this author
Ebright, YW More by this author
Ludescher, RD More by this author
Ebright, RH More by this author
Journal:
Journal of molecular biology
Volume:
312
Issue:
3
Pages:
453-468
Publication date:
2001-09-05
DOI:
EISSN:
1089-8638
ISSN:
0022-2836
URN:
uuid:32708572-e107-486b-9bb2-aee2a539803c
Source identifiers:
6397
Local pid:
pubs:6397

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