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Journal article

AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination.

Abstract:
RNA Polymerase II (Pol II) termination is dependent on RNA processing signals as well as specific terminator elements located downstream of the poly(A) site. One of the two major terminator classes described so far is the Co-Transcriptional Cleavage (CoTC) element. We show that homopolymer A/T tracts within the human β-globin CoTC-mediated terminator element play a critical role in Pol II termination. These short A/T tracts, dispersed within seemingly random sequences, are strong terminator elements, and bioinformatics analysis confirms the presence of such sequences in 70% of the putative terminator regions (PTRs) genome-wide.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/gks1335

Authors

More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author


More from this funder
Funding agency for:
Proudfoot, N


Publisher:
Oxford University Press
Journal:
Nucleic acids research More from this journal
Volume:
41
Issue:
3
Pages:
1797-1806
Publication date:
2013-02-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048


Language:
English
Keywords:
Pubs id:
369834
UUID:
uuid:31cd5546-c920-4e88-af28-426569ecbbf9
Local pid:
pubs:369834
Source identifiers:
369834
Deposit date:
2013-11-16
ARK identifier:

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