Journal article
Structural insights into the mechanism of protein transport by the Type 9 Secretion System translocon
- Abstract:
- Secretion systems are protein export machines that enable bacteria to exploit their environment through the release of protein effectors. The Type 9 Secretion System (T9SS) is responsible for protein export across the outer membrane (OM) of bacteria of the phylum Bacteroidota. Here we trap the T9SS of Flavobacterium johnsoniae in the process of substrate transport by disrupting the T9SS motor complex. Cryo-EM analysis of purified substrate-bound T9SS translocons reveals an extended translocon structure in which the previously described translocon core is augmented by a periplasmic structure incorporating the proteins SprE, PorD and a homologue of the canonical periplasmic chaperone Skp. Substrate proteins bind to the extracellular loops of a carrier protein within the translocon pore. As transport intermediates accumulate on the translocon when energetic input is removed, we deduce that release of the substrate–carrier protein complex from the translocon is the energy-requiring step in T9SS transport.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 21.8MB, Terms of use)
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- Publisher copy:
- 10.1038/s41564-024-01644-7
Authors
- Publisher:
- Springer Nature
- Journal:
- Nature Microbiology More from this journal
- Volume:
- 9
- Issue:
- 4
- Pages:
- 1089–1102
- Publication date:
- 2024-03-27
- Acceptance date:
- 2024-02-19
- DOI:
- EISSN:
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2058-5276
- Language:
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English
- Keywords:
- Pubs id:
-
1620406
- Local pid:
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pubs:1620406
- Deposit date:
-
2024-02-19
Terms of use
- Copyright holder:
- Lauber et al.
- Copyright date:
- 2024
- Rights statement:
- © The Author(s) 2024. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
- Licence:
- CC Attribution (CC BY)
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