Crystallographic analysis of the binding modes of thiazoloisoindolinone non-nucleoside inhibitors to HIV-1 reverse transcriptase and comparison with modeling studies.

Journal article

We have determined the crystal structures of thiazoloisoindolinone non-nucleoside inhibitors in complex with HIV-1 reverse transcriptase to high-resolution limits of 2.7 A (BM +21.1326) and 2. 52 A (BM +50.0934). We find that the binding modes of this series of inhibitors closely resemble that of "two-ring" non-nucleoside reverse transcriptase inhibitors. The structures allow rationalization of stereochemical requirements, structure-activity data, and drug resistance data. Comparisons with our previous structures suggest modifications to the inhibitors that might improve resilience to drug-resistant mutant forms of reverse transcriptase. Comparison with earlier modeling studies reveals that the predicted overlap of thiazoloisoindolinones with TIBO was largely correct, while that with nevirapine was significantly different.

Authors: Ren, J; Esnouf, R; Hopkins, A; Stuart, D; Stammers, D

• Publication status: Published
• Journal: Journal of medicinal chemistry
• Volume: 42
• Issue: 19
• Pages: 3845-3851
• Publication date: 1999-09-01
• DOI: 10.1021/jm990275t
• EISSN: 1520-4804
• ISSN: 0022-2623
• Language: English
• Keywords: Models, Molecular; Indoles; HIV Reverse Transcriptase; Thiazoles; Crystallography, X-Ray; Protein Conformation; Reverse Transcriptase Inhibitors; Protein Binding; Structure-Activity Relationship; Models, Chemical