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Catalysed amplification of faradaic shotgun tagging in ultrasensitive electrochemical immunoassays

Abstract:
We introduce a novel electrochemical protein quantitation based on the shotgun biotin tagging of proteins prior to their interfacial immunocapture and polymeric enzyme tagging. The highly amplified faradaic signals generated from a novel ferrocene-tyramine adduct enable fg mL-1 (attomolar) levels of detection and span cross a 5 orders of magnitude dynamic range. This work supports ultrasensitive protein marker detection in a single antibody immunoassay format.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1039/d2cc03509j

Authors

More by this author
Institution:
University of Oxford
Role:
Author
ORCID:
0000-0003-0618-945X
More by this author
Institution:
University of Oxford
Role:
Author
ORCID:
0000-0001-8567-0924
More by this author
Institution:
University of Oxford
Role:
Author
ORCID:
0000-0001-7734-1709


Publisher:
Royal Society of Chemistry
Journal:
Chemical Communications More from this journal
Volume:
58
Issue:
68
Pages:
9472-9475
Publication date:
2022-08-23
DOI:
EISSN:
1364-548X
ISSN:
1359-7345


Language:
English
Keywords:
Pubs id:
1275246
Local pid:
pubs:1275246
Source identifiers:
W4290754975
Deposit date:
2026-04-28
ARK identifier:
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