Journal article
Template-directed conjugation of heterogeneous oligonucleotides to a homobifunctional molecule for programmable supramolecular assembly
- Abstract:
- Nanoscience aspires to mimic nature's control over functional molecular assemblies. Here we present a templating technique for the efficient attachment of two different oligonucleotides to a homobifunctional molecule, enabling its controlled and programmable placement within a DNA nanostructure. We demonstrate its application to a range of organic molecules with different conjugation chemistries and water solubilities. We show that the two oligonucleotide adapters can be used to integrate a bifunctional cyanine dye into a self-assembled 3D-DNA origami nanostructure, giving control of both position and orientation. We also demonstrate the use of both adapters to exert dynamic control over the environment of the target molecule by means of a series of strand-displacement reactions.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 576.1KB, Terms of use)
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(Preview, Supplementary materials, pdf, 8.0MB, Terms of use)
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- Publisher copy:
- 10.1039/D2NR00197G
Authors
- Publisher:
- Royal Society of Chemistry
- Journal:
- Nanoscale More from this journal
- Volume:
- 14
- Issue:
- 12
- Pages:
- 4463-4468
- Publication date:
- 2022-02-28
- Acceptance date:
- 2022-02-28
- DOI:
- EISSN:
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2040-3372
- ISSN:
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2040-3364
- Language:
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English
- Keywords:
- Pubs id:
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1241711
- Local pid:
-
pubs:1241711
- Deposit date:
-
2022-02-28
Terms of use
- Copyright holder:
- Royal Society of Chemistry
- Copyright date:
- 2022
- Rights statement:
- © Royal Society of Chemistry 2022.
- Notes:
-
This is the accepted manuscript version of the article. The final version is available from Royal Society of Chemistry at https://doi.org/10.1039/D2NR00197G
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