Crystallization and preliminary X-ray analysis of three serotypes of foot-and-mouth disease virus.

Journal article

Foot-and-mouth disease viruses from serotypes O, A and C have been crystallized. The particular strains studied include O1K, A10(61), A22 Iraq 24/64, A24 Cruzeiro and C-S8c1. In addition, crystals have been grown of G67, a monoclonal antibody neutralization escape mutant derived from O1K, and of virus R100, recovered after the establishment of a persistent infection in baby hamster kidney cells with C-S8c1. Empty particles, capsids which lack the RNA genome, have also been crystallized for subtypes A22 Iraq 24/64 and A10(61). In almost all cases, crystals suitable for high resolution structure determination were obtained from (NH4)2SO4 or mixtures of polyethylene glycol and NH4Cl.

Authors: Curry, S; Abu-Ghazaleh, R; Blakemore, W; Fry, E; Jackson, T

• Publication status: Published
• Journal: Journal of molecular biology
• Volume: 228
• Issue: 4
• Pages: 1263-1268
• Publication date: 1992-12-01
• DOI: 10.1016/0022-2836(92)90332-e
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: Aphthovirus; Trypsin; Capsid; Genetic Variation; Serotyping; Mutation; Crystallization; X-Ray Diffraction; Virion