Annexin 1: differential expression in tumor and mast cells in human larynx cancer.

Journal article

Annexin 1 protein (ANXA1) expression was evaluated in tumor and mast cells in human larynx cancer and control epithelium. The effect of the exogenous ANXA1 (peptide Ac 2-26) was also examined during the cellular growth of the Hep-2 human larynx epidermoid carcinoma cell line. This peptide inhibited the proliferation of the Hep-2 cells within 144 hr. In surgical tissue specimens from 20 patients with larynx cancer, ultrastructural immunocytochemistry analysis showed in vivo down-regulation of ANXA1 expression in the tumor and increased in mast cells and Hep-2 cells treated with peptide Ac2-26. Combined in vivo and in vitro analysis demonstrated that ANXA1 plays a regulatory role in laryngeal cancer cell growth. We believe that a better understanding of the regulatory mechanisms of ANXA1 in tumor and mast cells may lead to future biological targets for the therapeutic intervention of human larynx cancer.

Authors: Silistino-Souza, R; Rodrigues-Lisoni, F; Cury, P; Maniglia, J; Raposo, L

• Publication status: Published
• Journal: International journal of cancer. Journal international du cancer
• Volume: 120
• Issue: 12
• Pages: 2582-2589
• Publication date: 2007-06-01
• DOI: 10.1002/ijc.22639
• EISSN: 1097-0215
• ISSN: 0020-7136
• Language: English
• Keywords: Tryptases; Reverse Transcriptase Polymerase Chain Reaction; Mast Cells; Laryngeal Neoplasms; Time Factors; Gene Expression Profiling; Microscopy, Immunoelectron; Aged; Epithelial Cells; Cell Line, Tumor; Peptides; Aged, 80 and over; Male; RNA, Messenger; Immunohistochemistry; Annexin A1; Humans; Larynx; Middle Aged; Gene Expression