Journal article
JmjC catalysed histone H2a N -methyl arginine demethylation and C4-arginine hydroxylation reveals importance of sequence-reactivity relationships
- Abstract:
- 2-Oxoglutarate (2OG) dependent Nε-methyl lysine demethylases (JmjC-KDMs) regulate eukaryotic transcription. We report studies showing that isolated forms of all human KDM4 and KDM5 JmjC enzymes catalyse demethylation of N-methylated Arg-3 of histone H2a. Unexpectedly, the results reveal that KDM4E and, less efficiently, KDM4D catalyse C-4 hydroxylation of Arg-20 of H2a on peptides, recombinant H2a, and calf histone extracts, including when the Arg-20 guanidino group is N-methylated. Combined with previous observations, our biochemical results highlight the importance of sequence context in determining the relative efficiencies of lysine and arginine demethylation reactions catalysed by KDM4s and KDM5s. At least in some cases changes in sequence can also enable a different JmjC reaction mode, such as C-4 arginine hydroxylation instead of demethylation. Further work is thus required to define the full scope of JmjC catalysed reactions in cells.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.9MB, Terms of use)
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- Publisher copy:
- 10.1038/s42003-024-07183-5
Authors
+ European Research Council
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- Funder identifier:
- https://ror.org/0472cxd90
- Programme:
- H2020 Priority Excellent Science
+ Engineering and Physical Sciences Research Council
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- Funder identifier:
- https://ror.org/0439y7842
- Publisher:
- Springer Nature
- Journal:
- Communications Biology More from this journal
- Volume:
- 7
- Issue:
- 1
- Article number:
- 1583
- Publication date:
- 2024-11-27
- Acceptance date:
- 2024-10-31
- DOI:
- EISSN:
-
2399-3642
- Language:
-
English
- Pubs id:
-
2067274
- Local pid:
-
pubs:2067274
- Source identifiers:
-
2453905
- Deposit date:
-
2024-11-27
Terms of use
- Copyright holder:
- Bonnici et al.
- Copyright date:
- 2024
- Rights statement:
- © The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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