Journal article
Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics
- Abstract:
-
SpyTag is a peptide that forms a spontaneous amide bond with its protein partner SpyCatcher. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures. We initially developed SpyTag and SpyCatcher by rational design, through splitting a domain from a Gram-positive bacterial adhesin. Here we establish a phage display platform able to select for specific amidation, achieving an order ...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Authors
Funding
+ European Research Council
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Funding agency for:
Keeble, A
Grant:
ERC-2013-CoG 615945-PeptidePadlock
+ European Research Council
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Funding agency for:
Banerjee, A
Grant:
ERC-2013-CoG 615945-PeptidePadlock
+ Engineering and Physical Sciences Research Council
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Funding agency for:
Ferla, M
Grant:
EP/N023226/1
+ European Research Council
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Funding agency for:
Reddington, S
Grant:
ERC-2013-CoG 615945-PeptidePadlock
+ Yayasan Khazanah, Oxford Centre for Islamic Studies
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Funding agency for:
Khairil Anuar, I
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Bibliographic Details
- Publisher:
- Wiley Publisher's website
- Journal:
- Angewandte Chemie International Edition Journal website
- Volume:
- 56
- Issue:
- 52
- Pages:
- 16521-16525
- Publication date:
- 2017-10-10
- Acceptance date:
- 2017-10-02
- DOI:
- EISSN:
-
1521-3773
- ISSN:
-
1433-7851
- Source identifiers:
-
735067
Item Description
- Pubs id:
-
pubs:735067
- UUID:
-
uuid:2b78d10b-9152-4d03-bc06-745c4b768f2e
- Local pid:
- pubs:735067
- Deposit date:
- 2017-10-11
Terms of use
- Copyright holder:
- © 2017 WILEY-VCH Verlag GmbH & Co KGaA, Weinheim
- Copyright date:
- 2017
- Notes:
- This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited
- Licence:
- CC Attribution (CC BY)
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