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Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics

Abstract:

SpyTag is a peptide that forms a spontaneous amide bond with its protein partner SpyCatcher. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures. We initially developed SpyTag and SpyCatcher by rational design, through splitting a domain from a Gram-positive bacterial adhesin. Here we establish a phage display platform able to select for specific amidation, achieving an order ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1002/anie.201707623

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
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Funding agency for:
Reddington, S
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Publisher:
Wiley Publisher's website
Journal:
Angewandte Chemie International Edition Journal website
Volume:
56
Issue:
52
Pages:
16521-16525
Publication date:
2017-10-10
Acceptance date:
2017-10-02
DOI:
EISSN:
1521-3773
ISSN:
1433-7851
Pubs id:
pubs:735067
URN:
uri:2b78d10b-9152-4d03-bc06-745c4b768f2e
UUID:
uuid:2b78d10b-9152-4d03-bc06-745c4b768f2e
Local pid:
pubs:735067
Paper number:
52

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