Abnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart.

Journal article

This study examined cardiac function and glucose metabolism in the 6-month-old db/db mouse, a model of type-2 diabetes. Cine magnetic resonance spectroscopy (MRI) was used to measure cardiac function in vivo. The db/db mice had decreased heart rates (17%, p<0.01) and stroke volumes (21%, p<0.05) that resulted in lower cardiac output (35%, p<0.01) than controls. Although there was no difference in ejection fraction between the 2 groups, db/db mouse hearts had a 35% lower maximum rate of ejection (p<0.01) than controls. In a protocol designed to assess maximal insulin-independent glucose uptake, hearts were isolated and perfused in Langendorff mode and subjected to 0.75 mL.min(-1).(g wet mass)(-1) low flow ischemia for 32 min. Glucose uptake during ischemia was 21% lower than in controls, and post-ischemic recovery of cardiac function was decreased by 30% in db/db mouse hearts (p<0.05). Total cardiac GLUT 4 protein was 56% lower (p<0.01) in db/db mice than in controls. In summary, the db/db mouse has abnormal left ventricular function in vivo, with impaired glucose uptake during ischemia, leading to increased myocardial damage.

Authors: Panagia, M; Schneider, J; Brown, B; Cole, M; Clarke, K

• Publication status: Published
• Journal: Canadian journal of physiology and pharmacology
• Volume: 85
• Issue: 3-4
• Pages: 289-294
• Publication date: 2007-01-01
• DOI: 10.1139/y07-028
• EISSN: 1205-7541
• ISSN: 0008-4212
• Language: English
• Keywords: Insulin; Glucose Transporter Type 4; Diabetes Mellitus, Type 2; Fatty Acids; Triglycerides; 3-Hydroxybutyric Acid; Heart; Myocardium; Mice, Inbred Strains; Heart Rate; Stroke Volume; Ischemia; Mice; Blood Glucose; Male; Cardiac Output; Animals