Journal article
Decline in pneumococcal vaccine serotype carriage, multiple-serotype carriage, and carriage density in Nepalese children after PCV10 introduction: a pre-post comparison study
- Abstract:
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Background: Carriage studies are an efficient means for assessing pneumococcal conjugate vaccine effect in settings where pneumococcal disease surveillance programmes are not well established. In this study the effect of 10-valent pneumococcal conjugate vaccine (PCV10) introduction on pneumococcal carriage and density among Nepalese children using a bacterial microarray and qPCR was examined.
Methods: PCV10 was introduced into the Nepalese infant immunisation schedule in August 2015. Nasopharyngeal swabs were collected from healthy Nepalese children in Kathmandu between April 2014 and December 2021. Samples were plated on blood agar, incubated overnight, and DNA extracted from plate sweeps. Pneumococcal serotyping was done using the Senti-SPv1.5 microarray (BUGS Bioscience, UK). DNA was extracted from swab media and qPCR performed for pneumococcal autolysin (lytA).
Results: A significant decline in prevalence of PCV10 serotypes was observed when comparing pre-PCV10 with post-PCV10 collection periods (36.5 %, 454/1244 vs 10.3 %, 243/2353, p < 0.0001). Multiple-serotype carriage was also observed to significantly decline when comparing pre-PCV10 with post-PCV10 periods (31.4 %, 390/ 1244 vs 22.2 %, 522/2353, p < 0.0001). Additionally, a significant decline in median neumococcal density was observed when comparing pre-PCV10 with post-PCV10 periods (3.3 vs 3.25 log10 GE/ml, p = 0.0196).
Conclusions: PCV10 introduction was associated with reduced, prevalence of all PCV10 serotypes, multiple serotype carriage, and pneumococcal carriage density.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 506.8KB, Terms of use)
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- Publisher copy:
- 10.1016/j.vaccine.2024.05.018
Authors
- Publisher:
- Elsevier
- Journal:
- Vaccine More from this journal
- Volume:
- 42
- Issue:
- 19
- Pages:
- 4066-4071
- Place of publication:
- Netherlands
- Publication date:
- 2024-05-23
- Acceptance date:
- 2024-05-11
- DOI:
- EISSN:
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1873-2518
- ISSN:
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0264-410X
- Pmid:
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38789369
- Language:
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English
- Keywords:
- Pubs id:
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1999052
- Local pid:
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pubs:1999052
- Deposit date:
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2024-06-04
Terms of use
- Copyright holder:
- Kandasamy et al.
- Copyright date:
- 2024
- Rights statement:
- © 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- Licence:
- CC Attribution (CC BY)
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