Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres.

Journal article

Mutations in human doublecortin (DCX) and knockdown of Dcx in rodents cause radial migration defects in the embryonic cerebral cortex. However, the brain phenotype of Dcx knockout mice is largely normal suggesting that Dcx is not necessary for most migration events. Adult subventricular zone (SVZ) cells migrate tangentially in the rostral migratory stream to the olfactory bulbs. Dcx is expressed in the SVZ but it is unknown if it is necessary for migration. We show that Dcx RNAi reduced SVZ cell migration in vitro, both cell autonomously and non-cell autonomously. Overexpression of Dcx rescued migration after knockdown, but did not increase migration by itself. Thus, Dcx is necessary not only for embryonic radial migration but also migration of adult SVZ cells.

Authors: Ocbina, P; Dizon, M; Shin, L; Szele, F

• Publication status: Published
• Journal: Molecular and cellular neurosciences
• Volume: 33
• Issue: 2
• Pages: 126-135
• Publication date: 2006-10-01
• DOI: 10.1016/j.mcn.2006.06.014
• EISSN: 1095-9327
• ISSN: 1044-7431
• Language: English
• Keywords: Mice, Knockout; Spheroids, Cellular; Phenotype; Cell Movement; Lateral Ventricles; Neuropeptides; Mice; Olfactory Bulb; 3' Untranslated Regions; Humans; Hela Cells; Animals; Microscopy, Video; Microtubule-Associated Proteins; RNA, Messenger; Brain; Cerebral Cortex; Age Factors; RNA Interference; Male