Journal article
Genome sequencing reveals metabolic and cellular interdependence in an amoeba-kinetoplastid symbiosis
- Abstract:
- Endosymbiotic relationships between eukaryotic and prokaryotic cells are common in nature. Endosymbioses between two eukaryotes are also known; cyanobacterium-derived plastids have spread horizontally when one eukaryote assimilated another. A unique instance of a non-photosynthetic, eukaryotic endosymbiont involves members of the genus Paramoeba, amoebozoans that infect marine animals such as farmed fish and sea urchins. Paramoeba species harbor endosymbionts belonging to the Kinetoplastea, a diverse group of flagellate protists including some that cause devastating diseases. To elucidate the nature of this eukaryote-eukaryote association, we sequenced the genomes and transcriptomes of Paramoeba pemaquidensis and its endosymbiont Perkinsela sp. The endosymbiont nuclear genome is ~9.5 Mbp in size, the smallest of a kinetoplastid thus far discovered. Genomic analyses show that Perkinsela sp. has lost the ability to make a flagellum but retains hallmark features of kinetoplastid biology, including polycistronic transcription, trans-splicing, and a glycosome-like organelle. Mosaic biochemical pathways suggest extensive 'cross-talk' between the two organisms, and electron microscopy shows that the endosymbiont ingests amoeba cytoplasm, a novel form of endosymbiont-host communication. Our data reveal the cell biological and biochemical basis of the obligate relationship between Perkinsela sp. and its amoeba host, and provide a foundation for understanding pathogenicity determinants in economically important Paramoeba.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 4.6MB, Terms of use)
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- Publisher copy:
- 10.1038/s41598-017-11866-x
Authors
+ Canadian Institutes of Health Research
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- Funding agency for:
- Archibald, J
- Grant:
- MOP-115141
+ Wellcome Trust
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- Funding agency for:
- Dean, S
- Gull, K
- Grant:
- 104627/Z/14/Z
- 104627/Z/14/Z
- Publisher:
- Nature Publishing Group
- Journal:
- Scientific Reports More from this journal
- Volume:
- 7
- Issue:
- 11688
- Pages:
- 1-8
- Publication date:
- 2017-09-15
- Acceptance date:
- 2017-08-31
- DOI:
- EISSN:
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2045-2322
- ISSN:
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2045-2322
- Pmid:
-
28916813
- Language:
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English
- Keywords:
- Pubs id:
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pubs:729967
- UUID:
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uuid:2ace8191-71b0-4ef6-b199-e0615ef40964
- Local pid:
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pubs:729967
- Source identifiers:
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729967
- Deposit date:
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2017-11-20
Terms of use
- Copyright holder:
- © Tanifuji, et al 2017
- Copyright date:
- 2017
- Notes:
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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