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Plasmodium falciparum-specific memory B-cell and antibody responses are associated with immunity in children living in an endemic area of Kenya

Abstract:
Identifying the mechanism of naturally acquired immunity against Plasmodium falciparum malaria could contribute to the design of effective malaria vaccines. Using a recently developed multiplexed FluoroSpot assay, we assessed cross-sectional pre-existing memory B-cells (MBCs) and antibody responses against six well known P. falciparum antigens (MSP-119, MSP-2 (3D7), MSP-2 (FC27), MSP-3, AMA-1 and CSP) and measured their associations with previous infections and time to clinical malaria in the ensuing malaria season in Kenyan children. These children were under active weekly surveillance for malaria as part of a long-term longitudinal malaria immunology cohort study, where they are recruited from birth. After performing Cox regression analysis, we found that children with a breadth of three or more antigen-specific MBC or antibody responses at the baseline had a reduced risk for malaria in the ensuing P. falciparum transmission season. Specifically, MBC responses against AMA-1, MSP-2 (3D7) and MSP-3, as well as antibody responses to MSP-2 (3D7) and MSP-3 were prospectively associated with a reduced risk for malaria. The magnitude or breadth of MBC responses were however not correlated with the cumulative number of malaria episodes since birth. We conclude that increased breadth for merozoite antigen-specific MBC and antibody responses is associated with protection against malaria.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3389/fimmu.2022.799306

Authors

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Role:
Author
ORCID:
0000-0002-6138-690X


Publisher:
Frontiers Media
Journal:
Frontiers in Immunology More from this journal
Volume:
13
Article number:
799306
Publication date:
2022-03-09
Acceptance date:
2022-02-16
DOI:
EISSN:
1664-3224
Pmid:
35355994


Language:
English
Keywords:
Pubs id:
1257046
Local pid:
pubs:1257046
Deposit date:
2022-06-10
ARK identifier:

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