External validation of the APPS, a new and simple outcome prediction score in patients with the acute respiratory distress syndrome

Journal article

AbstractWe have isolated and characterized a rat brain cDNA clone which encodes a new protein of 474 amino acids in length which contains two C2 domains structurally homologous to those present in synaptotagmins. The overall amino acid identity in C2 domains between this protein and the synaptotagmins is 36–44%. This protein also contains 3 putative consensus sequences for phosphorylation by cAMP-dependent protein kinase. RNA blot hybridization revealed a 3.0 kb transcript abundantly expressed only in the rat brain and the kidney. Thus, we called this brain/kidney protein (B/K). In situ hybridization and Northern blot analyses showed that the B/K transcript was found in forebrain including the olfactory bulb, cerebral cortex, hippocampus, and hypothalamus. In the kidney, high levels of B/K transcript were expressed in the papillary region of the inner medulla, the inner stripe of the outer medulla and the cortex. The selective expression in forebrain and kidney suggests that B/K may be involved in similar cAMP-dependent processes at these very different sites

Authors: Bos, LD; Schouten, LR; Cremer, OL; Ong, DSY; Schultz, MJ

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: SpringerOpen
• Journal: Annals of Intensive Care
• Volume: 6
• Issue: 1
• Pages: 89-89
• Publication date: 2016-09-14
• DOI: 10.1186/s13613-016-0190-0
• EISSN: 2110-5820
• ISSN: 2110-5820
• Language: English
• Keywords: Mechanical ventilation; Cohort; Anesthesiology; Area under the curve; Anesthesia; Emergency medicine; Internal medicine; Plateau pressure; Confidence interval; Receiver operating characteristic; Fraction of inspired oxygen; Predictive value of tests; Cutoff; ARDS; Lung; Acute respiratory distress; Medicine