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Journal article

PCR for capsular typing of Haemophilus influenzae.

Abstract:

A PCR method for the unequivocal assignment of Haemophilus influenzae capsular type (types a to f) was developed. PCR primers were designed from capsule type-specific DNA sequences cloned from the capsular gene cluster of each of the six capsular types. PCR product was amplified only from the capsular type for which the primers were designed. Product was confirmed by using either an internal oligonucleotide or restriction endonuclease digestion. A total of 172 H. influenzae strains of known c...

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Journal:
Journal of clinical microbiology
Volume:
32
Issue:
10
Pages:
2382-2386
Publication date:
1994-10-05
EISSN:
1098-660X
ISSN:
0095-1137
URN:
uuid:2a58e346-026d-479b-b5d4-c4410323af3c
Source identifiers:
92700
Local pid:
pubs:92700

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