Targeted microbubbles detected with high-frequency ultrasound can establish the molecular expression of blood vessels with submillimeter resolution. To improve microbubble-specific imaging at high frequencies, the subharmonic and second harmonic signal from individual microbubbles were measured as a function of size and pressure. Single phospholipid-shell microbubbles (1.1 to 5.0 microm in diameter) bound to gelatin, co-aligned with an optical microscope and transducer, were insonated with 30...Expand abstract
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Nonlinear emission from individual bound microbubbles at high frequencies.
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