Autocrine deactivation of macrophages in transgenic mice constitutively overexpressing IL-10 under control of the human CD68 promoter.

Journal article

IL-10 plays an essential role in blocking cytokine production by activated macrophages. To analyze the consequences of enforced expression of IL-10 by macrophages on innate and adaptive immune responses, we generated transgenic mice (macIL-10tg mice) expressing an epitope-tagged IL-10 (Flag-IL-10) under control of the human CD68 promoter. Expression of Flag-IL-10 was constitutive and restricted to macrophages, as shown by sorting splenocyte cell populations and intracellular staining for IL-1...

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Authors: Lang, R; Rutschman, R; Greaves, D; Murray, P

• Publication status: Published
• Journal: Journal of Immunology
• Volume: 168
• Issue: 7
• Pages: 3402-3411
• Publication date: 2002-04-01
• DOI: 10.4049/jimmunol.168.7.3402
• EISSN: 1550-6606
• ISSN: 0022-1767
• Language: English
• Keywords: Antigens, CD; Tumor Necrosis Factor-alpha; Autocrine Communication; Macrophages; Cell Count; Tuberculosis; Promoter Regions, Genetic; Epitopes, T-Lymphocyte; Lipopolysaccharides; Myeloid Cells; Macrophage Activation; Mice; Recombinant Fusion Proteins; Colony Count, Microbial; Interferon-gamma; Epitopes, B-Lymphocyte; Peptides; Animals; Female; Mice, Transgenic; Mycobacterium bovis; Cells, Cultured; Antigens, Differentiation, Myelomonocytic; Injections, Intraperitoneal; Gene Dosage; Signal Transduction; Humans; Male; Interleukin-10; Infertility, Male; Lymphoid Tissue; Interleukin-12