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Journal article

Low charge and reduced mobility of membrane protein complexes has implications for calibration of collision cross section measurements

Abstract:

Ion mobility mass spectrometry of integral membrane proteins provides valuable insights into their architecture and stability. Here we show that, due to their lower charge, the average mobility of native-like membrane protein ions is approximately 30% lower than that of soluble proteins of similar mass. This has implications for drift time measurements, made on traveling wave ion mobility mass spectrometers, which have to be calibrated to extract collision cross sections (Ω). Common calibrati...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1021/acs.analchem.6b00691

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More from this funder
Funding agency for:
Landreh, M
Grant:
Junior Research Fellowship
More from this funder
Funding agency for:
Landreh, M
Grant:
Junior Research Fellowship
More from this funder
Funding agency for:
Robinson, C
Grant:
Research Professorship
More from this funder
Funding agency for:
Benesch, J
Grant:
University Research Fellow
More from this funder
Funding agency for:
Robinson, C
Grant:
Research Professorship
Publisher:
American Chemical Society Publisher's website
Journal:
Analytical Chemistry Journal website
Volume:
88
Issue:
11
Pages:
5879–5884
Publication date:
2016-05-06
Acceptance date:
2016-05-06
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
Source identifiers:
623124
Language:
English
Pubs id:
pubs:623124
UUID:
uuid:297f1416-2458-4b49-acd2-a85bee393a86
Local pid:
pubs:623124
Deposit date:
2016-05-23

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