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Single molecule measurements of F1-ATPase reveal an interdependence between the power stroke and the dwell duration.

Abstract:

Increases in the power stroke and dwell durations of single molecules of Escherichia coli F(1)-ATPase were measured in response to viscous loads applied to the motor and inhibition of ATP hydrolysis. The load was varied using different sizes of gold nanorods attached to the rotating gamma subunit and/or by increasing the viscosity of the medium using PEG-400, a noncompetitive inhibitor of ATPase activity. Conditions that increase the duration of the power stroke were found to cause 20-fold in...

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Publication status:
Published

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Publisher copy:
10.1021/bi9008215

Authors


Spetzler, D More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
Hornung, T More by this author
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Journal:
Biochemistry
Volume:
48
Issue:
33
Pages:
7979-7985
Publication date:
2009-08-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:27c7209d-2d45-43b5-b544-36ced2c56250
Source identifiers:
154468
Local pid:
pubs:154468

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