An accessible, efficient and global approach for the large-scale sequencing of bacterial genomes

Journal article

We have developed an efficient and inexpensive pipeline for streamlining large-scale collection and genome sequencing of bacterial isolates. Evaluation of this method involved a worldwide research collaboration focused on the model organism Salmonella enterica, the 10KSG consortium. Following the optimization of a logistics pipeline that involved shipping isolates as thermolysates in ambient conditions, the project assembled a diverse collection of 10,419 isolates from low- and middle-income countries. The genomes were sequenced using the LITE pipeline for library construction, with a total reagent cost of less than USD$10 per genome. Our method can be applied to other large bacterial collections to underpin global collaborations.

Authors: Perez-Sepulveda, BM; Heavens, D; Pulford, CV; Predeus, AV; Low, R

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: BioMed Central
• Journal: Genome Biology
• Volume: 22
• Issue: 1
• Pages: 349-349
• Article number: 349
• Publication date: 2021-12-21
• DOI: 10.1186/s13059-021-02536-3
• EISSN: 1474-760X
• ISSN: 1474-7596
• Language: English
• Keywords: DNA sequencing; DNA; Genetics; Evolutionary biology; Genome Biology; Bacterial genome size; Personal genomics; Biology; Human genetics; Genome; Gene; Computational genomics; Genomics; Computational biology; Scale (ratio)