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Protamine 1 to protamine 2 ratio correlates with dynamic aspects of DNA fragmentation in human sperm.

Abstract:

OBJECTIVE: To investigate the relationship between the protamine 1 to protamine 2 (P1/P2) ratio and the rate of sperm DNA fragmentation in sperm samples from human males with proven fertility and three different cohorts of male patients. DESIGN: P1/P2 ratio was analyzed using acid-urea polyacrylamide acid-urea gels electrophoresis (PAGE). Sperm DNA fragmentation using sperm chromatin dispersion methodology was analyzed after 0, 4, 8, and 24 hours of incubation at 37°C. SETTING: University me...

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Publication status:
Published

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Authors


García-Peiró, A More by this author
Martínez-Heredia, J More by this author
Oliver-Bonet, M More by this author
Amengual, MJ More by this author
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Journal:
Fertility and sterility
Volume:
95
Issue:
1
Pages:
105-109
Publication date:
2011-01-05
DOI:
EISSN:
1556-5653
ISSN:
0015-0282
URN:
uuid:26e5102c-a5c5-4a34-954b-73007785fc73
Source identifiers:
248374
Local pid:
pubs:248374

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