Subunit exchange of multimeric protein complexes. Real-time monitoring of subunit exchange between small heat shock proteins by using electrospray mass spectrometry.

Journal article

The subunit exchange of the small heat shock proteins (sHSPs) PsHSP18.1 from pea and TaHSP16.9 from wheat has been monitored in real-time using nanoelectrospray mass spectrometry. By preserving the noncovalent interactions between subunits in the mass spectrometer, we show that these proteins are dodecameric. After mixing PsHSP18.1 and TaHSP16.9, a distribution of heterododecamers is formed. A comparison with spectra obtained from statistical modeling demonstrates that after equilibration the...

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Authors: Sobott, F; Benesch, J; Vierling, E; Robinson, C

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 277
• Issue: 41
• Pages: 38921-38929
• Publication date: 2002-10-01
• DOI: 10.1074/jbc.M206060200
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Models, Molecular; Spectrometry, Mass, Electrospray Ionization; Macromolecular Substances; Protein Binding; Plant Proteins; Triticum; Protein Subunits; Heat-Shock Proteins; Peas; Protein Structure, Quaternary