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Identifying bacterial airways infection in stable severe asthma using Oxford nanopore sequencing technologies

Abstract:

Previous metagenomic studies in asthma have been limited by inadequate sequencing depth for species-level bacterial identification and by heterogeneity in clinical phenotyping. We hypothesize that chronic bacterial airways infection is a key “treatable trait” whose prevalence, clinical phenotype and reliable biomarkers need definition. In this study, we have applied a method for Oxford Nanopore sequencing for the unbiased metagenomic characterization of severe asthma. We optimized methods to ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1128/spectrum.02279-21

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Oxford college:
New College
Role:
Author
Publisher:
American Society for Microbiology
Journal:
Microbiology Spectrum More from this journal
Volume:
10
Issue:
2
Article number:
e02279-21
Publication date:
2022-03-24
Acceptance date:
2022-03-02
DOI:
EISSN:
2165-0497
Language:
English
Keywords:
Pubs id:
1247018
Local pid:
pubs:1247018
Deposit date:
2022-03-24

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