DNA sequencing with MspA: Molecular Dynamics simulations reveal free-energy differences between sequencing and non-sequencing mutants

Journal article

MspA has been identified as a promising candidate protein as a component of a nanopore-based DNA-sequencing device. However the wildtype protein must be engineered to incorporate all of the features desirable for an accurate and efficient device. In the present study we have utilized atomistic molecular dynamics to perform umbrella-sampling calculations to calculate the potential of mean force (PMF) profiles for translocation of the four DNA nucleotides through MspA. We show there is an energetic barrier to translocation of individual nucleotides through a mutant that closely resembles the wildtype protein, but not through a mutant engineered for the purpose of sequencing. Crucially we are able to quantify the change in free energy for mutating key residues. Thus providing a quantitative characterisation of the energetic impact of individual amino acid sidechains on nucleotide translocation through the pore of MspA

Authors: Manara, RMA; Jayne Wallace, E; Khalid, S

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Nature Research
• Journal: Scientific Reports
• Volume: 5
• Issue: 1
• Pages: 12783-12783
• Publication date: 2015-08-10
• DOI: 10.1038/srep12783
• EISSN: 2045-2322
• ISSN: 2045-2322
• Language: English
• Keywords: Computational biology; DNA; DNA sequencing; Mutant; Physics; Dynamics (music); Biology; Genetics; Gene