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Comparative diagnostic performance of plasma microbial cell-free DNA sequencing versus amplicon-based blood next-generation sequencing in suspected bloodstream or endovascular infection: a single-center study

Abstract:
We compared plasma microbial cell-free DNA sequencing (mcfDNAseq) with an amplicon-based blood next-generation sequencing (NGS) assay for 10 patients with suspected bloodstream or endovascular infection. Plasma testing detected pathogens in eight (seven clinically meaningful), whereas amplicon testing was negative in all. Plasma mcfDNAseq provided higher test yield and overall sensitivity.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1017/ash.2026.10337

Authors

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Institution:
University of Oxford
Role:
Author
ORCID:
0000-0002-0333-1070


Publisher:
Cambridge University Press
Journal:
Antimicrobial Stewardship & Healthcare Epidemiology More from this journal
Volume:
6
Issue:
1
Article number:
e69
Publication date:
2026-03-27
Acceptance date:
2026-02-25
DOI:
EISSN:
2732-494X
ISSN:
2732-494X


Language:
English
Pubs id:
2407710
Local pid:
pubs:2407710
Source identifiers:
3893151
Deposit date:
2026-03-27
ARK identifier:
This ORA record was generated from metadata provided by an external service. It has not been edited by the ORA Team.

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